Centre for Cancer Research

Protein Kinase R publications

2007
Alirezaei M, Watry DD, Flynn CF, Kiosses WB, Masliah E, Williams BRG, Kaul M, Lipton SA and Fox HS (2007) Human immunodeficiency virus-1/surface glycoprotein 120 induces apoptosis through RNA-activated protein kinase signaling in neurons. J Neurosci 27(41):11047-11055.

Austin BA, Halford WP, Williams BRG and Carr DJJ (2007) Oligoadenylate synthetase/protein kinase R pathways and α/β TCR⁺ T cells are required for adenovirus vector: IFN-γ inhibition of Herpes simplex virus-1 in cornea. J Immunol 178(8):5166-5172.

Carpentier PA, Williams BR and Miller SD (2007) Distinct roles of protein kinase R and toll-like receptor 3 in the activation of astrocytes by viral stimuli. Glia 55(3):239-252.

Sadler AJ and Williams BRG (2007) Structure and function of the protein kinase R. In: Current Topics in Microbiology and Immunology, Vol 316: Interferon: The 50^th Anniversary. PM Pitha, ed. Springer, Berlin, pp 253-292.

Silva AM, Wang D, Komar AA, Castilho BA and Williams BRG (2007) Salicylates trigger protein synthesis inhibition in a protein kinase R-like endoplasmic reticulum kinase-dependent manner. J Biol Chem 282(14):10164-10171.

2006
Austin BA, Halford W, Silverman RH, Williams BRG and Carr DJJ (2006) OAS and PKR are not required for the antiviral effect of Ad:IFN-γ against acute HSV-1 in primary trigeminal ganglia cultures. J Interferon Cytokine Res 26:220-225.

Bakheet T, Williams BRG and Khabar KSA (2006) ARED 3.0: The large and diverse AU-rich transcriptome. Nucleic Acids Res 34:D111-D114.

Chang K-S, Cai Z, Zhang C, Sen GC, Williams BRG and Luo G (2006) Replication of hepatitis C virus (HCV) RNA in mouse embryonic fibroblasts: protein kinase R (PKR)-dependent and PKR-independent mechanisms for controlling HCV RNA replication and mediating interferon actions. J Virol 80:7364-7374.

Gabel F, Wang D, Madern D, Sadler A, Dayie K, Zamanian Daryoush M, Schwahn D, Zaccai G, Lee X and Williams BRG (2006) Dynamic flexibility of double-stranded RNA activated PKR in solution. J Mol Biol 359:610-623.

Green J, Khabar KSA, Koo BCA, Williams BRG and Polyak SJ (2006) Stability of CXCL-8 and related AU-rich mRNAs in the context of hepatitis C virus replication in vitro. J Infect Dis 193:802-811.

Samuel MA, Whitby K, Keller BC, Marri A, Barchet W, Williams BRG, Silverman RH, Gale M Jr and Diamond MS (2006) PKR and RNase L contribute to protection against lethal West Nile virus infection by controlling early viral spread in the periphery and replication in neurons. J Virol 80:7009-7019.

2005
Al-khatib K, Williams BRG, Silverman RH, Halford W and Carr DJJ (2005) Dichotomy between survival and lytic gene expression in RNase L- and PKR-deficient mice transduced with an adenoviral vector expressing murine IFN-β following ocular HSV-1 infection. Exp Eye Res 80:167-173.

Carr DJJ, Tomanek L, Silverman RH, Campbell IL and Williams BRG (2005) RNA-dependent protein kinase is required for alpha-1 interferon transgene-induced resistance to genital herpes simplex virus type 2. J Virol 79:9341-9345.

Flodström-Tullberg M, Hultcrantz M, Stotland A, Maday A, Tsai D, Fine C, Williams B, Silverman RH and Sarvetnick N (2005) RNase L and double-stranded RNA-dependent protein kinase exert complementary roles in islet cell defense during Coxsackievirus infection. J Immunol 174:1171-1177.

Khabar KSA, Bakheet T and Williams BRG (2005) AU-rich transient response transcripts in the human genome: expressed sequence tag clustering and gene discovery approach. Genomics 85:165-175.

Marques JT, Rebouillat D, Ramana CV, Murakami J, Hill JE, Gudkov A, Silverman RH, Stark GR and Williams BRG (2005) Down-regulation of p53 by double-stranded RNA modulates the antiviral response. J Virol 79:11105-11114.

Smith JA, Schmechel SC, Williams BRG, Silverman RH and Schiff LA (2005) Involvement of the interferon-regulated antiviral proteins PKR and RNase L in reovirus-induced shutoff of cellular translation. J Virol 79:2240-2250.

Zhou Y, Chase BI, Whitmore M, Williams BRG and Zhou A (2005) Double-stranded RNA-dependent protein kinase (PKR) is downregulated by phorbol ester. FEBS J 272:1568-1576.

2004
Al-khatib K, Williams BRG, Silverman RH, Halford W and Carr DJJ (2004) Distinctive roles for 2',5'-oligoadenylate synthetases and double-stranded RNA-dependent protein kinase R in the in vivo antiviral effect of an adenoviral vector expressing murine IFN-β. J Immunol 172:5638-5647.

Gorchakov R, Frolova E, Williams BRG, Rice CM and Frolov I (2004) PKR-dependent and -independent mechanisms are involved in translational shutoff during Sindbis virus infection. J Virol 78:8455-8467.

Raghavan A, Dhalla M, Bakheet T, Ogilvie RL, Vlasova IA, Khabar KSA, Williams BRG and Bohjanen PR (2004) Patterns of coordinate down-regulation of ARE-containing transcripts following immune cell activation. Genomics 84:1002-1013.

Silva AM, Whitmore M, Xu Z, Jiang Z, Li X and Williams BRG (2004) Protein kinase R (PKR) interacts with and activates mitogen-activated protein kinase kinase 6 (MKK6) in response to double-stranded RNA stimulation. J Biol Chem 279:37670-37676.

Xu Z, Wang D, Lee X and Williams BRG (2004) Biochemical analyses of multiple fractions of PKR purified from Escherichia coli. J Interferon Cytokine Res 24:522-535.

2003
Al-khatib K, Williams BRG, Silverman RH, Halford W and Carr DJJ (2003) The murine double-stranded RNA-dependent protein kinase PKR and the murine 2’, 5’-oligoadenylate synthetase-dependent RNase L are required for IFN-b-mediated resistance against herpes simplex virus type 1 in primary trigeminal ganglion culture. Virology 313:126-135.

Bakheet T, Williams BR and Khabar KS (2003) ARED 2.0: an update of AU-rich element mRNA database. Nucleic Acids Res 31:421-423.

Frevel MAE, Bakheet T, Silva AM, Hissong JG, Khabar KSA and Williams BRG (2003) p38 mitogen-activated protein kinase-dependent and -independent signaling of mRNA stability of AU-rich element-containing transcripts. Mol Cell Biol 23:425-436.

Jiang Z, Zamanian-Daryoush M, Nie H, Silva AM, Williams BRG and Li X (2003) Poly(dI·dC)-induced Toll-like receptor 3 (TLR3)-mediated activation of NFκB and MAP kinase is through an interleukin-1 receptor-associated kinase (IRAK)-independent pathway employing the signaling components TLR3-TRAF6-TAK1-TAB2-PKR. J Biol Chem 278:16713-16719.

Khabar KSA, Siddiqui YM, al-Zoghaibi F, al-Haj L, Dhalla M, Zhou A, Dong B, Whitmore M, Paranjape J, Al-Ahdal MN, Al-Mohanna F, Williams BRG and Silverman RH (2003) RNase L mediates transient control of interferon response through modulation of the double-stranded RNA-dependent protein kinase PKR. J Biol Chem 278:20124-20132.

López CB, García-Sastre A, Williams BRG and Moran TM (2003) Type I IFN induction pathway, but not released interferon, participates in the maturation of dendritic cells induced by negative-strand RNA viruses. J Infect Dis 187:1126-1136.

Navarro A, Frevel M, Gamero AM, Williams BRG, Feldman G and Larner AC (2003) Thrombomodulin RNA is destabilized through its 3’-untranslated element in cells exposed to IFN-γ. J Interferon Cytokine Res 23:723-728.

Sawicki DL, Silverman RH, Williams BRG and Sawicki SG (2003) Alphavirus minus-strand synthesis and persistence in mouse embryo fibroblasts derived from mice lacking RNase L and protein kinase R. J Virol 77:1801-1811.

Tebo J, Der S, Frevel M, Khabar KSA, Williams BRG and Hamilton TA (2003) Heterogeneity in control of mRNA stability by AU-rich elements. J Biol Chem 278:12085-12093.

Yin Z, Haynie J, Williams BRG and Yang Y-C (2003) C114 is a novel IL-11-inducible nuclear double-stranded RNA-binding protein that inhibits protein kinase R. J Biol Chem 278:22838-22845.

2002
Al-khatib K, Williams BRG, Silverman RH, Halford WP and Carr DJJ (2002) Absence of PKR attenuates the anti-HSV-1 activity of an adenoviral vector expressing murine IFN-b. J Interferon Cytokine Res 22:861-871.

Guidotti LG, Morris A, Mendez H, Koch R, Silverman RH, Williams BRG and Chisari FV (2002) Interferon-regulated pathways that control hepatitis B virus replication in transgenic mice. J Virol 76:2617-2621.

Härle P, Cull V, Agbaga M-P, Silverman R, Williams BRG, James C and Carr DJJ (2002) Differential effect of murine alpha/beta interferon transgenes on antagonization of herpes simplex virus type I replication. J Virol 76:6558-6567.

Toshchakov V, Jones BW, Perera P-Y, Thomas K, Cody MJ, Zhang S, Williams BRG, Major J, Hamilton TA, Fenton MJ and Vogel SN (2002) TLR4, but not TLR2, mediates IFN-β-induced STAT1α/β-dependent gene expression in macrophages. Nat Immunol 3:392-398.

Wang X, Basler CF, Williams BRG, Silverman RH, Palese P and García-Sastre A (2002) Functional replacement of the carboxy-terminal two-thirds of the influenza A virus NS1 protein with short heterologous dimerization domains. J Virol 76:12951-12962.

Xiang Y, Condit RC, Vijaysri S, Jacobs B, Williams BRG and Silverman RH (2002) Blockade of interferon induction and action by the E3L double-stranded RNA binding proteins of vaccinia virus. J Virol 76:5251-5259.

2001
Bakheet T, Frevel M, Williams BRG, Greer W and Khabar KSA (2001) ARED: human AU-rich element-containing mRNA database reveals an unexpectedly diverse functional repertoire of encoded proteins. Nucleic Acids Res 29:246-254.

Deb A, Haque SJ, Mogensen T, Silverman RH and Williams BRG (2001) RNA-dependent protein kinase PKR is required for activation of NF-kB by IFN-g in a STAT1-independent pathway. J Immunol 166:6170-6180.

Deb A, Zamanian-Daryoush M, Xu Z, Kadereit S and Williams BRG (2001) Protein kinase PKR is required for platelet-derived growth factor signaling of c-fos gene expression via Erks and Stat3. EMBO J 20:2487-2496.

2000
Bandyopadhyay SK, de la Motte CA and Williams BRG (2000) Induction of E-selectin expression by double-stranded RNA and TNF-α is attenuated in murine aortic endothelial cells derived from double-stranded RNA-activated kinase (PKR)-null mice. J Immunol 164:2077-2083.

Cai R, Carpick B, Chun RF, Jeang K-T and Williams BRG (2000) HIV-1 TAT inhibits PKR activity by both RNA-dependent and RNA-independent mechanisms. Arch Biochem Biophys 373:361-367.

Goh KC, deVeer MJ and Williams BRG (2000) The protein kinase PKR is required for p38 MAPK activation and the innate immune response to bacterial endotoxin. EMBO J 19:4292-4297.

Iordanov MS, Paranajape JM, Zhou A, Wong J, Williams BRG, Meurs EF, Silverman RH and Magun BE (2000) Activation of p38 mitogen-activated protein kinase and c-Jun NH₂-terminal kinase by double-stranded RNA and encephalomyocarditis virus: involvement of RNase L, protein kinase R, and alternative pathways. Mol Cell Biol 20:617-627.

Kadereit S, Xu H, Engeman TM, Yang Y-L, Fairchild RL and Williams BRG (2000) Negative regulation of CD8⁺ T cell function by the IFN-induced and double-stranded RNA-activated kinase PKR. J Immunol 165:6896-6901.

Khabar KSA, Dhalla M, Siddiqui Y, Zhou A, Al-Ahdal MN, Der SD, Silverman RH and Williams BRG (2000) Effect of deficiency of the double-stranded RNA-dependent protein kinase, PKR, on antiviral resistance in the presence or absence of ribonuclease L: HSV-1 replication is particularly sensitive to deficiency of the major IFN-mediated enzymes. J Interferon Cytokine Res 20:653-659.

Leib DA, Machalek MA, Williams BRG, Silverman RH and Virgin HW (2000) Specific phenotypic restoration of an attenuated virus by knockout of a host resistance gene. Proc Natl Acad Sci USA 97:6097-6101.

Nanduri S, Rahman F, Williams BRG and Qin J (2000) A dynamically tuned double-stranded RNA binding mechanism for the activation of antiviral kinase PKR. EMBO J 19:5567-5574.

Uetani K, Der SD, Zamanian-Daryoush M, de la Motte C, Lieberman BY, Williams BRG and Erzurum SC (2000) Central role of double-stranded RNA-activated protein kinase in microbial induction of nitric oxide synthase. J Immunol 165: 988-996.

Xu Z and Williams BRG (2000) The B56a regulatory subunit of protein phosphatase 2A is a target for regulation by the double-stranded RNA-dependent protein kinase PKR. Mol Cell Biol 20:5285-5299.

Zamanian-Daryoush M, Mogensen TH, DiDonato JA and Williams BRG (2000) NF-_KB activation by double-stranded-RNA-activated protein kinase (PKR) is mediated through NF-κB-inducing kinase and IκB kinase. Mol Cell Biol 20:1278-1290.